Lateralized expression of left-right axis formation genes is shared by adult brains of lefty and righty scale-eating cichlids

Yuichi Takeuchi, Asano Ishikawa, Yoichi Oda, Jun Kitano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Variation in the laterality often exists within species and can be maintained by frequency-dependent selection. Although the molecular developmental mechanisms underlying the left-right axis formation have been investigated, the genomic mechanisms underlying variation in laterality remain largely unknown. The scale-eating cichlid Perissodus microlepis in Lake Tanganyika exhibit lateralized predation; lefty individuals with the mouth opening toward the right preferentially attack on the prey's left trunk, while righty individuals with the opposite opening attacks on the right trunk. Here, we performed RNA-sequencing and subsequent confirmation with quantitative-PCR in the telencephalon, optic tectum, and hindbrain of the cichlid and identified five genes (pkd1b, ntn1b, ansn, pde6g, and rbp4l1) that were differentially expressed between the hemispheres regardless of the laterality. Surprisingly, pkd1b and ntn1b are involved in nodal and netrin signalling, respectively, which are important for left–right asymmetry formation during early embryogenesis. This result indicates that nodal- and netrin-related signals may also play important roles in the maintenance of asymmetry in adult brain. By contrast, no genes showed reversal of lateral differences between lefty and righty individuals in any brain regions examined, suggesting that laterality in the scale-eating cichlid does not simply result from inversion of the left–right asymmetry of gene expression.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume28
DOIs
Publication statusPublished - 2018 Dec
Externally publishedYes

Keywords

  • Brain lateralization
  • Cichlid
  • Gene expression
  • Laterality
  • Left–right asymmetry
  • RNA-seq

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Lateralized expression of left-right axis formation genes is shared by adult brains of lefty and righty scale-eating cichlids'. Together they form a unique fingerprint.

  • Cite this