Lateral resolution enhancement of laser scanning microscopy by a higher-order radially polarized mode beam

Yuichi Kozawa, Terumasa Hibi, Aya Sato, Hibiki Horanai, Makoto Kurihara, Nobuyuki Hashimoto, Hiroyuki Yokoyama, Tomomi Nemoto, Shunichi Sato

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

We demonstrate that the lateral resolution of confocal laser scanning microscopy is dramatically improved by a higher-order radially polarized (HRP) beam with six concentric rings. This beam was generated simply by inserting liquid crystal devices in front of an objective lens. An HRP beam visualized aggregated 0.17 μm beads individually and is also applicable to biological imaging. This method can extend the capability of conventional laser scanning microscopes without modification of the system, with the exception of the addition of the liquid crystal devices in the optical path.

Original languageEnglish
Pages (from-to)15947-15954
Number of pages8
JournalOptics Express
Volume19
Issue number17
DOIs
Publication statusPublished - 2011 Aug 15

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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