Laser trapping and Raman spectroscopy of single cellular organelles in the nanometer range

Katsuhio Ajito, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

The laser trapping technique combined with near-infrared Raman (NIR) spectroscopy was used for the analysis of single cellular organelles in the nanometer range. The samples were synaptosomes, nerve-ending particles (about 500-700 nm in diameter) isolated from a neuron in a rat brain, dispersed in the phosphate buffer solution. The NIR laser Raman trapping (NIR-LRT) system trapped a single synaptosome without photochemical damage and provided a Raman spectrum of the sample with less fluorescence background. After the background subtraction from the Raman spectrum, two large peaks appeared, which are attributed to the peaks of the CH2 deformation mode and the amide I mode. This indicates the laser-trapped synaptosomes include some types of lipids and proteins. The result demonstrates that the NIR-LRT system can determine biological molecules in single cellular organelles in the nanometer range. Further improvement of the detection sensitivity will enable us to get detailed information about the functions of single cellular organelles in the brain, which will be valuable for neuroscience.

Original languageEnglish
Pages (from-to)11-14
Number of pages4
JournalLab on a Chip
Volume2
Issue number1
DOIs
Publication statusPublished - 2002 Feb 1
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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