Laser-dephosphorylation of phosphogelatin and its indirect quantitative analysis using FT-IR

Katsunoti Ishii, Yoshihisa Ninomiya, Minori Takegoshi, Toshihiro Kushibiki, Sachiko Yoshihashi-Suzuki, Masaya Yamamoto, Yasuhiko Tabata, Kunio Awazu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Phosphorylation and dephosphorylation are considered to be important reactions that control the active and inactive factors of proteins. In regenerative medicine of the osteoconnective tissue (a tendon, a ligament), it has been reported that the biomaterial possessing phosphate groups promote formation of HAP, the main component of hard tissues. The noncontact measurement of phosphate groups and low-destructive controlling of phosphate groups allow for the accurate regeneration of the osteoconnective tissue, and the validation. Our objective is to propose the nondestructive controlling and measuring method of phosphorylation for regenerative medicine. In this study, as the indirect quantitative analysis of phosphate groups, we examine the correlation between the mid-infrared absorbance ratio and the ratio of phosphate groups introduction theoretically calculated from a colorimetric determination method. And the noncontact controlling method of the quantities of phosphate groups, we examine the selective and low-destructive bond cutting of phosphate groups in the phosphogelatin using a mid-infrared laser.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
Publication statusPublished - 2006 May 8
Externally publishedYes
EventOptical Interactions with Tissue and Cells XVII - San Jose, CA, United States
Duration: 2006 Jan 232006 Jan 25

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6084
ISSN (Print)0277-786X

Other

OtherOptical Interactions with Tissue and Cells XVII
CountryUnited States
CitySan Jose, CA
Period06/1/2306/1/25

Keywords

  • FT-IR
  • Free Electron Laser
  • Laser-dephosphorylation
  • Molybdenum blue method
  • Phosphate ester
  • Phosphate group
  • Phosphogelatin
  • Regenerative medicine

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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