Large scale structural analysis of cDNAs in the model legume, Lotus japonicus

Erika Asamizu, Masao Watanabe, Satoshi Tabata

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Lotus japonicus possesses certain characteristics suited to molecular genetic and genomic analyses and has been adopted as a model species in the study of legume plants. To make a catalogue of genes expressed in L. japonicus and understand biological processes specific to legume plants, large scale EST analyses have been performed. To date, more than 26,000 EST sequences of L. japonicus have been deposited in the public databases. These sequences were developed by five laboratories using different organs. In this review, information obtained from two EST projects carried out in Japan is presented. Some 7137 non-redundant EST groups from young plants and 718 groups from flower buds were generated. A similarity search revealed that homologues of nodulin genes in other legume plants, as well as genes related to secondary metabolism, seed development and the reproductive process, were included in the EST collection, indicating the usefulness of the EST clones in the study of biological phenomena distinctive to legume plant species.

Original languageEnglish
Pages (from-to)451-455
Number of pages5
JournalJournal of Plant Research
Volume113
Issue number4
DOIs
Publication statusPublished - 2000 Dec
Externally publishedYes

Keywords

  • EST
  • Legume
  • Lotus japonicus
  • cDNA

ASJC Scopus subject areas

  • Plant Science

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