TY - JOUR
T1 - Large-Scale Preparation of Giant Vesicles by Squeezing a Lipid-Coated Marshmallow-like Silicone Gel in a Buffer
AU - Hayase, Gen
AU - Nomura, Shin Ichiro M.
N1 - Funding Information:
We would like to thank Prof. M. Yanagisawa for helpful advice. This research was supported by the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) (Nos. 24104004, 15H02774, and 15K17909) and by the Japan Agency for Medical Research and Development (AMED)−Core Research for Evolutional Science and Technology (CREST) (16gm0810001h0102).
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/9/18
Y1 - 2018/9/18
N2 - Giant vesicles were efficiently produced by squeezing a lipid (l-α-phosphatidylcholine from egg yolk)-coated marshmallow-like flexible macroporous silicone monolith in a buffer. The mean diameter of the obtained vesicles was 2 μm, showing a wide distribution, up to tens of micrometers, which was similar to that of vesicles formed by a natural swelling method. It was possible to prepare vesicle dispersions on a scale from several microliters to several hundred milliliters. A protein synthesis system (PURE system) contained in vesicles prepared using this method functioned effectively. Our absorbing-squeezing method is expected to help in studies that use giant vesicles such as artificial cells and drug delivery systems.
AB - Giant vesicles were efficiently produced by squeezing a lipid (l-α-phosphatidylcholine from egg yolk)-coated marshmallow-like flexible macroporous silicone monolith in a buffer. The mean diameter of the obtained vesicles was 2 μm, showing a wide distribution, up to tens of micrometers, which was similar to that of vesicles formed by a natural swelling method. It was possible to prepare vesicle dispersions on a scale from several microliters to several hundred milliliters. A protein synthesis system (PURE system) contained in vesicles prepared using this method functioned effectively. Our absorbing-squeezing method is expected to help in studies that use giant vesicles such as artificial cells and drug delivery systems.
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U2 - 10.1021/acs.langmuir.8b01801
DO - 10.1021/acs.langmuir.8b01801
M3 - Article
C2 - 30149718
AN - SCOPUS:85053239686
VL - 34
SP - 11021
EP - 11026
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 37
ER -