Large-scale, multicenter study of cerebrospinal fluid tau protein phosphorylated at serine 199 for the antemortem diagnosis of Alzheimer's disease

Nobuo Itoh, Hiroyuki Arai, Katsuya Urakami, Koichi Ishiguro, Hideto Ohno, Harald Hampel, Katharina Buerger, Jens Wiltfang, Markus Otto, Hans Kretzschmar, Hans Juergen Moeller, Masaki Imagawa, Hideki Kohno, Kenji Nakashima, Shigeki Kuzuhara, Hidetada Sasaki, Kazutomo Imahori

Research output: Contribution to journalArticlepeer-review

183 Citations (Scopus)

Abstract

We surveyed a total of 570 cerebrospinal fluid (CSF) samples from a variety of diseases, including Alzheimer's disease (AD; n = 236), non-AD-demented and nondemented diseases (n = 239), and normal controls (n = 95) to quantitate levels of tau protein phosphorylated at serine 199 (CSF/phospho-tau199) by a recently established sandwich ELISA. The CSF/phospho-tau199levels in the AD group were significantly elevated compared to those in all the other non-AD groups. Receiver operating characteristics curves showed that the diagnostic sensitivity and specificity for the AD group vs all the other non-AD groups using the CSF/phospho-tau199were 85.2% and 85.0%, respectively. Furthermore, there was a significant positive correlation between CSF/phospho-tau199and CSF/total-tau levels in the AD group. Elevated CSF/phospho-tau199in the AD group was noted irrespective of age, gender, dementia severity, and number of apolipoprotein E4 alleles. Thus, we suggest that CSF/phospho-tau199may be a novel and logical biomarker in supporting antemortem diagnosis of AD.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalAnnals of Neurology
Volume50
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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