Large-scale collection and characterization of promoters of human and mouse genes

Yutaka Suzuki, Riu Yamashita, Matsuyuki Shirota, Yuta Sakakibara, Joe Chiba, Junko Mizushima-Sugano, Alexander E. Kel, Takahiro Arakawa, Piero Carninci, Jun Kawai, Yoshihide Hayashizaki, Toshihisa Takagi, Kenta Nakai, Sumio Sugano

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We report the generation and initial characterization of a large-scale collection of sequences of putative promoter regions (PPRs) of human and mouse genes. Based on our unique collection of 400,225 and 580,209 human and mouse full-length cDNAs, we determined exact transcriptional start sites (TSSs). Using positional information of the TSSs, we could retrieve adjacent sequences as PPRs for 8,793 and 6,875 human and mouse genes, respectively. The positions of the PPRs were 4 kb upstream to previously reported 5'-ends of cDNAs on average, demonstrating that full-length cDNA information is indispensable for this purpose. Among those PPRs supported by experimentally validated TSSs, 3,324 could be paired as mutually homologous genes between human and mouse and were used for the comprehensive comparative studies. The sequence identities in the proximal regions of the TSSs were 45% on average, and 22,794 putative transcription factor binding sites that are conserved between human and mouse were identified. The data resource created in the present work and the results of the sequences' initial characterization should lay the firm foundation for deciphering the transcriptional modulations of human genes. All the data were deposited and made available through a database for comparative studies, DBTSS.

Original languageEnglish
Pages (from-to)429-444
Number of pages16
JournalIn silico biology
Volume4
Issue number4
Publication statusPublished - 2004 Dec 1

Keywords

  • Comparative genomics
  • Full-length cDNA
  • Promoter
  • Transcriptional start sites

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Computational Mathematics
  • Computational Theory and Mathematics

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  • Cite this

    Suzuki, Y., Yamashita, R., Shirota, M., Sakakibara, Y., Chiba, J., Mizushima-Sugano, J., Kel, A. E., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Takagi, T., Nakai, K., & Sugano, S. (2004). Large-scale collection and characterization of promoters of human and mouse genes. In silico biology, 4(4), 429-444.