Lanthanide-based protease activity sensors for time-resolved fluorescence measurements

Shin Mizukami; Kazuhiro Tonai; Masahiro Kaneko; Kazuya Kikuchi

doi:10.1021/ja800322b

Lanthanide-based protease activity sensors for time-resolved fluorescence measurements

Shin Mizukami, Kazuhiro Tonai, Masahiro Kaneko, Kazuya Kikuchi

Research output: Contribution to journal › Article › peer-review

106 Citations (Scopus)

Abstract

Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from proteins or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.

Original language	English
Pages (from-to)	14376-14377
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	130
Issue number	44
DOIs	https://doi.org/10.1021/ja800322b
Publication status	Published - 2008 Nov 5
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja800322b

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abstract = "Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from proteins or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.",

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AU - Tonai, Kazuhiro

AU - Kaneko, Masahiro

AU - Kikuchi, Kazuya

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Y1 - 2008/11/5

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AB - Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from proteins or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.

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Lanthanide-based protease activity sensors for time-resolved fluorescence measurements

Abstract

ASJC Scopus subject areas

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