Laminin inhibits Cl--secretion across canine tracheal epithelium

M. Yamamoto, M. Yamaya, S. Shimura, M. Satoh, T. Sasaki, K. Shirato

Research output: Contribution to journalArticlepeer-review


We examined the effect of laminin (a major component of the basement membrane) on potential difference (PD) and short circuit current (SCC) in both posterior epithelial membranes (native tissue) and cultured epithelial cell layers from canine trachea using an Ussing chamber. Although laminin itself did not alter the baseline values of SCC or PD, it significantly inhibited in a dose-dependent manner the isoproterenol induced SCC and PD rises of the epithelial membrane. In the cultured epithelial layer treated with amiloride, laminin did not alter the baseline values of SCC, PD or resistance (R) but it significantly inhibited the isoproterenol induced SCC rises. However, laminin did not significantly inhibit the bradykinin induced SCC rise in the cultured epithelial layer treated with amiloride. These findings indicate that laminin is a determinant in ion transport (mainly Cl- secretion) across canine tracheal epithelium, inhibiting the β-agonist induced Cl- transport. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalRespiration Physiology
Issue number3
Publication statusPublished - 1998 Dec
Externally publishedYes


  • Epithelium, tracheal, bioelectric properties
  • Mammals, dog
  • Membrane, epithelium, laminin
  • Protein, laminin
  • Upper airways, tracheal epithelium, laminin

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine


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