TY - JOUR
T1 - Laminin inhibits Cl--secretion across canine tracheal epithelium
AU - Yamamoto, M.
AU - Yamaya, M.
AU - Shimura, S.
AU - Satoh, M.
AU - Sasaki, T.
AU - Shirato, K.
N1 - Funding Information:
We gratefully acknowledge Brent Bell for reading the manuscript. This study was supported by a grant-in-aid for General Scientific Research (07457144) from the Japanese Ministry of Education, Science and Culture.
PY - 1998/12
Y1 - 1998/12
N2 - We examined the effect of laminin (a major component of the basement membrane) on potential difference (PD) and short circuit current (SCC) in both posterior epithelial membranes (native tissue) and cultured epithelial cell layers from canine trachea using an Ussing chamber. Although laminin itself did not alter the baseline values of SCC or PD, it significantly inhibited in a dose-dependent manner the isoproterenol induced SCC and PD rises of the epithelial membrane. In the cultured epithelial layer treated with amiloride, laminin did not alter the baseline values of SCC, PD or resistance (R) but it significantly inhibited the isoproterenol induced SCC rises. However, laminin did not significantly inhibit the bradykinin induced SCC rise in the cultured epithelial layer treated with amiloride. These findings indicate that laminin is a determinant in ion transport (mainly Cl- secretion) across canine tracheal epithelium, inhibiting the β-agonist induced Cl- transport. Copyright (C) 1998 Elsevier Science B.V.
AB - We examined the effect of laminin (a major component of the basement membrane) on potential difference (PD) and short circuit current (SCC) in both posterior epithelial membranes (native tissue) and cultured epithelial cell layers from canine trachea using an Ussing chamber. Although laminin itself did not alter the baseline values of SCC or PD, it significantly inhibited in a dose-dependent manner the isoproterenol induced SCC and PD rises of the epithelial membrane. In the cultured epithelial layer treated with amiloride, laminin did not alter the baseline values of SCC, PD or resistance (R) but it significantly inhibited the isoproterenol induced SCC rises. However, laminin did not significantly inhibit the bradykinin induced SCC rise in the cultured epithelial layer treated with amiloride. These findings indicate that laminin is a determinant in ion transport (mainly Cl- secretion) across canine tracheal epithelium, inhibiting the β-agonist induced Cl- transport. Copyright (C) 1998 Elsevier Science B.V.
KW - Epithelium, tracheal, bioelectric properties
KW - Mammals, dog
KW - Membrane, epithelium, laminin
KW - Protein, laminin
KW - Upper airways, tracheal epithelium, laminin
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U2 - 10.1016/S0034-5687(98)00095-4
DO - 10.1016/S0034-5687(98)00095-4
M3 - Article
C2 - 9926991
AN - SCOPUS:0032449698
VL - 114
SP - 277
EP - 284
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
SN - 1569-9048
IS - 3
ER -