Lactobacillus reuteri LA6 and Lactobacillus gasseri LA39 isolated from faeces of the same human infant produce identical cyclic bacteriocin

Yasushi Kawai, Yasuyuki Ishii, Koichiro Uemura, Haruki Kitazawa, Tadao Saito, Takatoshi Itoh

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Reutericin 6, a bacteriocin produced by Lactobacillus reuteri LA6 that was isolated from the faeces of a human infant at 2 months of age, was purified to homogeneity from broth culture-supernatant by reverse-phase chromatography. Molecular weight (5652) by mass spectrometry and primary structure of reutericin 6 were identical to that of gassericin A produced by Lactobacillus gasseri LA39 which was isolated from the faeces of the same human infant at 4 months old. Reutericin 6 was shown to be a class II and cyclic bacteriocin. PCR amplification on the chromosome DNA of L reuteri LA6 as a template with the primers based on the DNA sequences cloned (gassericin A and acidocin B from Lactobacillus acidophilus M46) and sequencing revealed that L. reuteri LA6 has the structural gene for gassericin A with no variations among those primers. These results indicate that a bacteriocin of the same structure has been produced by different lactobacilli species isolated from the same infant.

Original languageEnglish
Pages (from-to)407-415
Number of pages9
JournalFood Microbiology
Volume18
Issue number4
DOIs
Publication statusPublished - 2001 Jan 1

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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