TY - JOUR
T1 - Lactobacillus delbrueckii crl 581 differentially modulates tlr3-triggered antiviral innate immune response in intestinal epithelial cells and macrophages
AU - Elean, Mariano
AU - Albarracin, Leonardo
AU - Fukuyama, Kohtaro
AU - Zhou, Binghui
AU - Tomokiyo, Mikado
AU - Kitahara, Shugo
AU - Araki, Shota
AU - Suda, Yoshihito
AU - Saavedra, Lucila
AU - Villena, Julio
AU - Hebert, Elvira M.
AU - Kitazawa, Haruki
N1 - Funding Information:
Funding: This study was supported by ANPCyT–FONCyT Grant PICT-2016-0410 to J.V. and PICT-2018-01378 and PIP 272 Grants to E.M.H. This study was supported by a Grant-in-Aid for Scientific Research (A) (19H00965) and Open Partnership Joint Projects of JSPS Bilateral Joint Research Projects from the Japan Society for the Promotion of Science (JSPS) to H.K. This research was supported by grants from the project of NARO Bio-oriented Technology Research Advancement Institution (research program on the development of innovative technology, No. 01002A) to H.K. This study was also supported by JSPS Core-to-Core Program, A. Advanced Research Networks titled Establishment of international agricultural immunology research-core for a quantum improvement in food safety.
Funding Information:
This study was supported by ANPCyT?FONCyT Grant PICT-2016-0410 to J.V. and PICT-2018-01378 and PIP 272 Grants to E.M.H. This study was supported by a Grant-in-Aid for Scientific Research (A) (19H00965) and Open Partnership Joint Projects of JSPS Bilateral Joint Research Projects from the Japan Society for the Promotion of Science (JSPS) to H.K. This research was supported by grants from the project of NARO Bio-oriented Technology Research Advancement Institution (research program on the development of innovative technology, No. 01002A) to H.K. This study was also supported by JSPS Core-to-Core Program, A. Advanced Research Networks titled Establishment of international agricultural immunology research-core for a quantum improvement in food safety.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.
AB - Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo. This study aimed to characterize further the immunomodulatory properties of the technologically relevant starter culture L. delbrueckii subsp. lactis CRL 581 by evaluating its interaction with intestinal epithelial cells and macrophages in the context of innate immune responses triggered by TLR3. Our results showed that the CRL 581 strain was able to adhere to porcine intestinal epithelial (PIE) cells and mucins. The CRL 581 strain also augmented the expression of antiviral factors (IFN-α, IFN-β, Mx1, OAS1, and OAS2) and reduced inflammatory cytokines in PIE cells triggered by TLR3 stimulation. In addition, the influence of L. delbrueckii subsp. lactis CRL 581 on the response of murine RAW macrophages to the activation of TLR3 was evaluated. The CRL 581 strain was capable of enhancing the expression of IFN-α, IFN-β, IFN-γ, Mx1, OAS1, TNF-α, and IL-1β. Of note, the CRL 581 strain also augmented the expression of IL-10 in macrophages. The results of this study show that the high proteolytic strain L. delbrueckii spp. lactis CRL 581 was able to beneficially modulate the intestinal innate antiviral immune response by regulating the response of both epithelial cells and macrophages relative to TLR3 activation.
KW - Antiviral response
KW - Immunobiotics
KW - Intestinal epithelial cells
KW - Lactobacillus delbrueckii subsp. lactis CRL 581
KW - Macrophages
KW - TLR3
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UR - http://www.scopus.com/inward/citedby.url?scp=85119984539&partnerID=8YFLogxK
U2 - 10.3390/microorganisms9122449
DO - 10.3390/microorganisms9122449
M3 - Article
AN - SCOPUS:85119984539
VL - 9
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 12
M1 - 2449
ER -