Capillary electrophoresis is being used increasingly for microsatellite genotyping. Here, we report that laboratory temperature greatly affects the estimated allele size, with 5°C changing the estimated allele size by up to 0.7 bp. With accurate room temperature control, this variation would be relatively unimportant but unfortunately, a questionnaire shows that daily and seasonal laboratory temperature differences are often substantial. One solution is to use a set of reference genotypes, which also greatly assists comparisons between different platforms.
- ABI 310
- Capillary electrophoresis
- Land snail
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)