Laboratory temperature variation is a previously unrecognized source of genotyping error during capillary electrophoresis

Angus Davison, Satoshi Chiba

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Capillary electrophoresis is being used increasingly for microsatellite genotyping. Here, we report that laboratory temperature greatly affects the estimated allele size, with 5°C changing the estimated allele size by up to 0.7 bp. With accurate room temperature control, this variation would be relatively unimportant but unfortunately, a questionnaire shows that daily and seasonal laboratory temperature differences are often substantial. One solution is to use a set of reference genotypes, which also greatly assists comparisons between different platforms.

Original languageEnglish
Pages (from-to)321-323
Number of pages3
JournalMolecular Ecology Notes
Volume3
Issue number2
DOIs
Publication statusPublished - 2003 Jun 1

Keywords

  • ABI 310
  • Capillary electrophoresis
  • Genotyping
  • Land snail
  • Microsatellite
  • Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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