Labeling of islet cells with iron oxide nanoparticles through DNA hybridization for highly sensitive detection by MRI

Narufumi Kitamura, Ryusuke Nakai, Haruyasu Kohda, Keiko Furuta-Okamoto, Hiroo Iwata

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A labeling method for islet cells with superparamagnetic iron oxide nanoparticles (SPIOs) based on DNA hybridization is proposed for monitoring of transplanted islets by magnetic resonance imaging (MRI). The surfaces of SPIOs were modified by via Michael reaction by reacting oligo-(deoxyadenylic acid)-bearing a terminal thiol group at the 5′-end ((dA)20-SH) with maleic acid functional groups on the SPIOs. The SPIOs were immobilized on islet cells which had been pretreated with oligo-(thymidylic acid)-poly(ethylene glycol)-phospholipid conjugates ((dT)20-PEG-DPPE) through DNA hybridization. Transmission electron microscopy observations revealed that SPIOs were initially anchored on the islet cell surfaces and subsequently transferred to endosomes or exfoliated with time. The SPIO-labeled islet cells could be clearly detected as dark spots by T2â̂ - -weighted MR image, whereas non-labeled islet cells could not be detected.

Original languageEnglish
Pages (from-to)7175-7181
Number of pages7
JournalBioorganic and Medicinal Chemistry
Volume21
Issue number22
DOIs
Publication statusPublished - 2013 Nov 15
Externally publishedYes

Keywords

  • Contrast agent
  • DNA hybridization
  • Islet
  • MRI
  • SPIO
  • Surface modification
  • ssDNA-PEG- phospholipid conjugates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Labeling of islet cells with iron oxide nanoparticles through DNA hybridization for highly sensitive detection by MRI'. Together they form a unique fingerprint.

Cite this