TY - JOUR
T1 - Label-free tracking of intracellular molecular crowding with cell-cycle progression using Raman microscopy
AU - Shibata, Daiki
AU - Kajimoto, Shinji
AU - Nakabayashi, Takakazu
N1 - Funding Information:
The authors thank Prof. N. Hirasawa and Dr. R. Segawa of Tohoku University for their kind support in the flow cytometry experiments. This work was supported by JSPS KAKENHI Grant Numbers JP17H05869 (TN), JP19H02666 (SK), and JP20H04689 (SK), and JST, PRESTO Grant Number JPMJPR20E5 (SK). SK also acknowledges Research grant (19E030) from Kurita Water and Environment Foundation (KWEF) in Japan.
Funding Information:
The authors thank Prof. N. Hirasawa and Dr. R. Segawa of Tohoku University for their kind support in the flow cytometry experiments. This work was supported by JSPS KAKENHI Grant Numbers JP17H05869 (TN), JP19H02666 (SK), and JP20H04689 (SK), and JST, PRESTO Grant Number JPMJPR20E5 (SK). SK also acknowledges Research grant (19E030) from Kurita Water and Environment Foundation (KWEF) in Japan.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/9/16
Y1 - 2021/9/16
N2 - Intracellular environments are highly crowded with biomolecules, called molecular crowding, and the structures and functions of biomolecules in cells are influenced by molecular crowding. In this study, we tracked the changes in intracellular crowding environments during cell cycle progression using Raman imaging. The intensity ratio between the C–H and O–H stretching bands allows us to quantitatively monitor the crowding environments. The results show that the nucleus is more crowded in the M phase than in other phases; however, the cytoplasm is maintained. The changes in molecular crowding in the nucleus can affect the stability and regulation of biomolecules.
AB - Intracellular environments are highly crowded with biomolecules, called molecular crowding, and the structures and functions of biomolecules in cells are influenced by molecular crowding. In this study, we tracked the changes in intracellular crowding environments during cell cycle progression using Raman imaging. The intensity ratio between the C–H and O–H stretching bands allows us to quantitatively monitor the crowding environments. The results show that the nucleus is more crowded in the M phase than in other phases; however, the cytoplasm is maintained. The changes in molecular crowding in the nucleus can affect the stability and regulation of biomolecules.
KW - Cell cycle
KW - Intracellular environment
KW - Molecular crowding
KW - Raman microscopy
KW - Water
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U2 - 10.1016/j.cplett.2021.138843
DO - 10.1016/j.cplett.2021.138843
M3 - Article
AN - SCOPUS:85108965735
VL - 779
JO - Chemical Physics Letters
JF - Chemical Physics Letters
SN - 0009-2614
M1 - 138843
ER -