Label-Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water

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17 Citations (Scopus)

Abstract

We propose a label-free method for measuring intracellular temperature using a Raman image of a cell in the O−H stretching band. Raman spectra of cultured cells and the medium were first measured at various temperatures using a Raman microscope and the intensity ratio of the two regions of the O−H stretching band was calculated. The intensity ratio varies linearly with temperature in both the medium and cells, and the resulting calibration lines allow simultaneous visualization of both intracellular and extracellular temperatures in a label-free manner. We applied this method to the measurement of temperature changes after the introduction of FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone) in living cells. We observed a temperature rise in the cytoplasm and succeeded in obtaining an image of the change in intracellular temperature after the FCCP treatment.

Original languageEnglish
Pages (from-to)7755-7760
Number of pages6
JournalAngewandte Chemie - International Edition
Volume59
Issue number20
DOIs
Publication statusPublished - 2020 May 11

Keywords

  • Raman spectroscopy
  • analytical methods
  • biophysics
  • thermochemistry
  • water chemistry

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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