Label-free detection of DNA hybridization with light-addressable potentiometric sensors: Comparison of various DNAimmobilization strategies

T. Bronder, C. S. Wu, A. Poghossian, C. F. Werner, M. Keusgend, M. J. Schöning

Research output: Contribution to journalConference articlepeer-review

11 Citations (Scopus)

Abstract

Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).

Original languageEnglish
Pages (from-to)755-758
Number of pages4
JournalProcedia Engineering
Volume87
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event28th European Conference on Solid-State Transducers, EUROSENSORS 2014 - Brescia, Italy
Duration: 2014 Sep 72014 Sep 10

Keywords

  • DNA hybridization
  • Field-effect sensor
  • LAPS
  • Lable-free detection

ASJC Scopus subject areas

  • Engineering(all)

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