Label-free detection and classification of DNA by surface vibration spectroscopy in conjugation with electrophoresis

Ko Ichiro Miyamoto, Ken Ichi Ishibashi, Kei Hiroi, Yasuo Kimura, Hisao Ishii, Michio Niwano

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We propose a method of in situ (in vitro) analysis of the chemical bonding state of DNA in aqueous solution by infrared absorption spectroscopy in the multiple internal reflection geometry (MIR-IRAS) in conjugation with electrophoresis. We demonstrate that MIR-IRAS is capable of detecting in vitro DNA hybridization. In our method, a Si prism, through which infrared lights penetrate and are also internally reflecting, also serves as the electrode for electrophoresis. We show that with a positive or negative potential being applied to the electrode (prism), DNA molecules are concentrated on or expelled from the electrode, respectively, leading to highly sensitive detection and manipulation of DNA in aqueous solution.

Original languageEnglish
Article number053902
Pages (from-to)1-3
Number of pages3
JournalApplied Physics Letters
Volume86
Issue number5
DOIs
Publication statusPublished - 2005 Jan 31

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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