Label-free biomedical imaging of hydrodynamics in single human cells

Hisanori Fukunaga, Hirofumi Yoshimura, Yukio Nishina, Yoji Nagashima, Masaru Tachibana

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Intracellular hydrodynamics is of considerable importance to regulate cellular functions. Using Raman microspectroscopy and imaging, we visualized the time-dependent changes of water concentration in single human acute promyelocytic leukemia HL-60 cells, and also showed the labelfree biomedical imaging of water molecules, lipids and proteins for analyzing molecular distribution. Taken together, our results demonstrated that we can distinguish between intracellular water and other major cell components, and that when the physiological status of cells changes with time, the Raman spectral intensities for intracellular water concentration also changes. Therefore, this biospectroscopic analysis of intracellular hydrodynamics can provide biomarkers to examine activities in dynamic living systems, and has significant potential in determining cell specificity for novel therapeutic approaches.

Original languageEnglish
Pages (from-to)177-181
Number of pages5
JournalBiomedical Research
Issue number3
Publication statusPublished - 2010 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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