Label-free and real time monitoring of adipocyte differentiation by surface infrared spectroscopy

Yuki Aonuma, Yasuhiko Kondo, Ayumi Hirano-Iwata, Atena Nishikawa, Yasuo Shinohara, Hiroo Iwata, Yasuo Kimura, Michio Niwano

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We have investigated in situ real-time monitoring of cell differentiation to adipocytes by using infrared absorption spectroscopy with the multiple internal reflection (MIR-IRAS) geometry. Preadipocyte 3T3-L1 cells were cultured on a silicon prism and their adipogenesis was monitored with MIR-IRAS for seven days. Differentiated cells showed a C=O stretching band of triacylglycerides, together with the protein amide I and amide II bands. The C=O stretching band appeared before optical microscopic confirmation of fat droplet formation, a typical morphological marker for adipocytes, demonstrating that MIR-IRAS is particularly useful for non-destructive monitoring of early adipogenesis. An effect of genistein, an antiadipogenic agent, was also investigated based on the peak intensity of the C=O stretching and amide bands. Thus, MIR-IRAS has a potential to evaluate antiadipogenic agents in terms of their effects on fat and protein synthesis during adipogenesis.

Original languageEnglish
Pages (from-to)1176-1182
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume176
DOIs
Publication statusPublished - 2013

Keywords

  • Adipogenesis
  • Infrared spectroscopy
  • Live cell monitoring

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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