L-Histidine decarboxylase protein and activity in rat brain microvascular endothelial cells

J. Yamakami, E. Sakurai, A. Kuramasu, E. Sakurai, Kazuhiko Yanai, T. Watanabe, Y. Tanaka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective and Design: L-Histidine decarboxylase (HDC) is the primary enzyme regulating histamine biosynthesis. This study was carried out to examine whether the cultured rat brain microvascular endothelial cells (BMEC), which constitute the blood-brain barrier (BBB), have the ability to form histamine, and whether HDC mRNA is expressed in rat BMEC. Material: Male, 3-week-old Wistar rats were used. For in vitro studies, rat BMEC were isolated from rat brains, and subculture cells were grown on collagen-coated culture flask and slide. Methods: HDC assay, immunofluorescence analysis and expression of HDC mRNA by RT-PCR were performed in rat BMEC. Results: The HDC activity of the BMEC was estimated to be 0.14 ± 0.05 p mol/min/mg protein. This activity was completely inhibited by (S)-α-fluoromethylhistidine, a specific inhibitor of HDC. Using a polyclonal anti HDC antibody and immunofluorescence microscopy, we confirmed the presence of HDC protein in rat BMEC. RT-PCR also showed the expression of HDC mRNA in rat BMEC. Conclusions: L-Histidine uptaken by rat BMEC was shown to be converted to histamine, suggesting that HDC plays an important role in BBB.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalInflammation Research
Volume49
Issue number5
DOIs
Publication statusPublished - 2000 Jun 26

Keywords

  • Blood-brain barrier
  • Histamine
  • L-Histidine decarboxylase
  • Rat brain microvascular endothelial cell

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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  • Cite this

    Yamakami, J., Sakurai, E., Kuramasu, A., Sakurai, E., Yanai, K., Watanabe, T., & Tanaka, Y. (2000). L-Histidine decarboxylase protein and activity in rat brain microvascular endothelial cells. Inflammation Research, 49(5), 231-235. https://doi.org/10.1007/s000110050584