L-glutamate as an excitatory transmitter at the neuromuscular junction of a beetle larva

Daisuke Yamamoto, Hiroshi Washio

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The effects of L-glutamate and acetylcholine on the ventral muscle fibres of the larval mealworm Tenebrio molitor were studied by means of microelectrodes. Bath application of L-glutamate at concentrations higher than 1 × 10 4M suppressed excitatory postsynaptic potentials (EPSPs) and evoked both a depolarisation and a reduction in the input resistance of the muscle fibre. In contrast, acetylcholine chloride (up to 1 mM) had no effect at all. Circumscribed spots could be detected on the fibre surface where iontophoretic applications of L-glutamate caused transient depolarizations (glutamate potentials). Focal extracellular recordings revealed that the glutamate sensitive spots were identical with synaptic sites. The reversal potentials of the EPSP and the L-glutamate potential were identical. These results are compatible with the hypothesis that L-glutamate is an excitatory transmitter at the neuromuscular junction.

    Original languageEnglish
    Pages (from-to)253-256
    Number of pages4
    JournalJournal of Insect Physiology
    Volume26
    Issue number4
    DOIs
    Publication statusPublished - 1980 Jan 1

    Keywords

    • L-glutamate potential
    • Tenebrio molitor
    • excitatory postsynaptic potential
    • excitatory transmitter

    ASJC Scopus subject areas

    • Physiology
    • Insect Science

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