l-Carbocisteine reduces neutrophil elastase-induced mucin production

Masanori Yasuo, Keisaku Fujimoto, Hitomi Imamura, Atsuhito Ushiki, Shintaro Kanda, Kenji Tsushima, Hiroshi Kubo, Mutsuo Yamaya, Keishi Kubo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Human neutrophil elastase (HNE) exists in high concentrations in airway secretions and produces mucus hypersecretion in patients with chronic obstructive pulmonary disease (COPD). l-Carbocisteine improves the quality of life and reduces exacerbation in COPD patients. However the precise mechanism is uncertain. We examined the effects of l-carbocisteine on HNE-induced mucus hypersecretion and on the production of reactive oxygen species (ROS) which is associated with mucin production induced by HNE. NCI-H292, a human lung mucoepidermoid carcinoma cell line, was treated with or without HNE and l-carbocisteine. MUC5AC mRNA expression and ROS production in the cells, and MUC5AC protein concentration in supernatants were measured. HNE increased MUC5AC mRNA expression and MUC5AC protein concentration in supernatants in the cells. l-Carbocisteine reduces HNE-induced mRNA expression and protein secretion of MUC5AC. l-Carbocisteine also reduced ROS production in the cells induced by HNE. Reduction of HNE-induced mucus secretion by l-carbocisteine in the pulmonary epithelial cells may partly relate to the reduction of ROS.

Original languageEnglish
Pages (from-to)214-216
Number of pages3
JournalRespiratory Physiology and Neurobiology
Volume167
Issue number2
DOIs
Publication statusPublished - 2009 Jun 30

Keywords

  • Antioxidant
  • Human neutrophil elastase
  • Mucin production
  • Oxidative stress
  • l-Carbocisteine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

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