L-Canavanine Resistance as a Positive Selectable Marker in Diploid Yeast Transformation through Integral Disruption of the CAN1 Gene

Tetsuyoshi Suizu, Yuzuru Iimura, Katsuya Gomi, Kojiro Takahashi, Shodo Hara, Kiyoshi Yoshizawa

Research output: Contribution to journalArticle

Abstract

We have developed a novel transformation system for yeasts carrying no selectable markers. By using a plasmid, PYHH-1, containing an internal region of the CAN1 gene, a wild type yeast strain was endowed with l-canavanine resistance (canl) through integral disruption of the resident CAN1 gene. Diploid strains as well as haploids can be transformed, indicating that l-canavanine resistance can be used as a positive selectable marker for the transformation of industrial yeasts.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalAgricultural and Biological Chemistry
Volume53
Issue number2
DOIs
Publication statusPublished - 1989 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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