L-canavanine resistance as a positive selectable marker in diploid yeast transformation through integral disruption of the can1 gene

Tetsuyoshi Suizu; Yuzuru Iimura; Katsuya Gomi; Kojiro Takahashi; Shodo Hara; Kiyoshi Yoshizawa

doi:10.1080/00021369.1989.10869329

L-canavanine resistance as a positive selectable marker in diploid yeast transformation through integral disruption of the can1 gene

Tetsuyoshi Suizu, Yuzuru Iimura, Katsuya Gomi, Kojiro Takahashi, Shodo Hara, Kiyoshi Yoshizawa

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have developed a novel transformation system for yeasts carrying no selectable markers. By using a plasmid, pYHH-1, containing an internal region of the CAN1 gene, a wild type yeast strain was endowed with L-canavanine resistance (canl) through integral disruption of the resident CAN1 gene. Diploid strains as well as haploids can be transformed, indicating that L-canavanine resistance can be used as a positive selectable marker for the transformation of industrial yeasts.

Original language	English
Pages (from-to)	431-436
Number of pages	6
Journal	Agricultural and Biological Chemistry
Volume	53
Issue number	2
DOIs	https://doi.org/10.1080/00021369.1989.10869329
Publication status	Published - 1989 Jan 1
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1080/00021369.1989.10869329

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L-canavanine resistance as a positive selectable marker in diploid yeast transformation through integral disruption of the can1 gene. / Suizu, Tetsuyoshi; Iimura, Yuzuru; Gomi, Katsuya; Takahashi, Kojiro; Hara, Shodo; Yoshizawa, Kiyoshi.

In: Agricultural and Biological Chemistry, Vol. 53, No. 2, 01.01.1989, p. 431-436.

Research output: Contribution to journal › Article › peer-review

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