TY - JOUR
T1 - L-canavanine resistance as a positive selectable marker in diploid yeast transformation through integral disruption of the can1 gene
AU - Suizu, Tetsuyoshi
AU - Iimura, Yuzuru
AU - Gomi, Katsuya
AU - Takahashi, Kojiro
AU - Hara, Shodo
AU - Yoshizawa, Kiyoshi
PY - 1989/1/1
Y1 - 1989/1/1
N2 - We have developed a novel transformation system for yeasts carrying no selectable markers. By using a plasmid, pYHH-1, containing an internal region of the CAN1 gene, a wild type yeast strain was endowed with L-canavanine resistance (canl) through integral disruption of the resident CAN1 gene. Diploid strains as well as haploids can be transformed, indicating that L-canavanine resistance can be used as a positive selectable marker for the transformation of industrial yeasts.
AB - We have developed a novel transformation system for yeasts carrying no selectable markers. By using a plasmid, pYHH-1, containing an internal region of the CAN1 gene, a wild type yeast strain was endowed with L-canavanine resistance (canl) through integral disruption of the resident CAN1 gene. Diploid strains as well as haploids can be transformed, indicating that L-canavanine resistance can be used as a positive selectable marker for the transformation of industrial yeasts.
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U2 - 10.1080/00021369.1989.10869329
DO - 10.1080/00021369.1989.10869329
M3 - Article
AN - SCOPUS:84885682861
VL - 53
SP - 431
EP - 436
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -