TY - JOUR
T1 - Kinin and histamine stimulate Cl- secretion in gerbil middle ear epithelium
T2 - Connection to otitis media
AU - Furukawa, Masayuki
AU - Suzuki, Hideaki
AU - Ikeda, Katsuhisa
AU - Oshima, Takeshi
AU - Yamaya, Mutsuo
AU - Sasaki, Hidetada
AU - Takasaka, Tomonori
N1 - Funding Information:
We are grateful to Dr. K. Yanai for kindly providing the histamine antagonists. We appreciate the excellent secretarial assistance of Y. Furukawa. This research was supported by Grant-in-Aid 09470367 (to K.I.) from the Ministry of Education, Science and Culture of Japan.
PY - 1999/6
Y1 - 1999/6
N2 - The effects of bradykinin (BK) and histamine on transepithelial ion transport in primary cultures of gerbil middle ear epithelium were investigated. Lysyl-bradykinin (lys-BK) elicited a transient increase in short-circuit current (I(sc)) when added to apical or basolateral surfaces. Lys-BK had a larger effect than BK or des-arg9-BK on both epithelial surfaces. Histamine induced a transient increase in I(sc) only when added to the basolateral surface. Mepyramine, an H1 histamine antagonist, greatly reduced the histamine-induced I(sc). The H2 and H3 histamine antagonists were both ineffective for inhibiting the I(sc) responses to histamine. Diphenylamine-2-carboxylate or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, Cl- channel blockers, significantly blocked the I(sc) responses to lys-BK or histamine. The Ca2+-mobilizing action of lys-BK and histamine was also investigated in single middle ear epithelial cells. BK and histamine induced an increase in the intracellular Ca1+ concentration. 1,2-Bis-(2- aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, a calcium chelator, greatly reduced the increase in the I(sc) responses to lys-BK or histamine. These data indicate that BK and histamine activate intracellular Ca2+-dependent mechanisms, leading to apical Cl- secretion in the cultured gerbil middle ear epithelium via B2 BK receptors and H1 histamine receptors.
AB - The effects of bradykinin (BK) and histamine on transepithelial ion transport in primary cultures of gerbil middle ear epithelium were investigated. Lysyl-bradykinin (lys-BK) elicited a transient increase in short-circuit current (I(sc)) when added to apical or basolateral surfaces. Lys-BK had a larger effect than BK or des-arg9-BK on both epithelial surfaces. Histamine induced a transient increase in I(sc) only when added to the basolateral surface. Mepyramine, an H1 histamine antagonist, greatly reduced the histamine-induced I(sc). The H2 and H3 histamine antagonists were both ineffective for inhibiting the I(sc) responses to histamine. Diphenylamine-2-carboxylate or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, Cl- channel blockers, significantly blocked the I(sc) responses to lys-BK or histamine. The Ca2+-mobilizing action of lys-BK and histamine was also investigated in single middle ear epithelial cells. BK and histamine induced an increase in the intracellular Ca1+ concentration. 1,2-Bis-(2- aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, a calcium chelator, greatly reduced the increase in the I(sc) responses to lys-BK or histamine. These data indicate that BK and histamine activate intracellular Ca2+-dependent mechanisms, leading to apical Cl- secretion in the cultured gerbil middle ear epithelium via B2 BK receptors and H1 histamine receptors.
KW - B bradykinin receptor
KW - H histamine receptor
KW - Intracellular Ca concentration
KW - Lysyl- bradykinin
KW - Short-circuit current
UR - http://www.scopus.com/inward/record.url?scp=0033054975&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033054975&partnerID=8YFLogxK
U2 - 10.1016/S0378-5955(99)00042-8
DO - 10.1016/S0378-5955(99)00042-8
M3 - Article
C2 - 10392553
AN - SCOPUS:0033054975
VL - 132
SP - 109
EP - 116
JO - Hearing Research
JF - Hearing Research
SN - 0378-5955
IS - 1-2
ER -