Kinetochore-microtubule interaction during S phase in Saccharomyces cerevisiae

Etsushi Kitamura, Kozo Tanaka, Yoko Kitamura, Tomoyuki U. Tanaka

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

In the budding yeast Saccharomyces cerevisiae, microtubule-organizing centers called spindle pole bodies (SPBs) are embedded in the nuclear envelope, which remains intact throughout the cell cycle (closed mitosis). Kinetochores are tethered to SPBs by microtubules during most of the cell cycle, including G1 and M phases; however, it has been a topic of debate whether microtubule interaction is constantly maintained or transiently disrupted during chromosome duplication. Here, we show that centromeres are detached from microtubules for 1-2 min and displaced away from a spindle pole in early S phase. These detachment and displacement events are caused by centromere DNA replication, which results in disassembly of kinetochores. Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are subsequently transported poleward by microtubules. Our study gives new insights into kinetochore-microtubule interaction and kinetochore duplication during S phase in a closed mitosis.

Original languageEnglish
Pages (from-to)3319-3330
Number of pages12
JournalGenes and Development
Volume21
Issue number24
DOIs
Publication statusPublished - 2007 Dec 15

Keywords

  • Closed mitosis
  • Kinetochore
  • Microtubule
  • S phase
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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