Kinetic study of interleukin‐2 binding on the reconstituted interleukin‐2 receptor complexes including the human γ chain

Mitsuo Matsuoka, Toshikazu Takeshita, Naoto Ishii, Masataka Nakamura, Takao Ohkubo, Kazuo Sugamura

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To gain an insight into roles of the interleukin‐2 (IL‐2) receptor γ chain in IL‐2 binding mechanisms, we examined association and dissociation rate constants for IL‐2 binding with a series of fibroblastoid L929 cell lines expressing IL‐2 receptor complexes reconstituted by transfection with the α, β and γ genes. The association rate constant (k) with the αβγ complex on L cells was fourfold larger than that with the αβ complex on L cells, and the dissociation rate constant (k') was one fifth of that with the αβ complex. These results indicate that the γ chain is involved in both mechanisms by which IL‐2 associates with and dissociates from receptors, resulting in the generation of the high‐affinity IL‐2 receptor along with the α and β chains. During the course of this study, we found that the IL‐2 dissociation from αβγ complex on lymphoid cells was a lot slower than that from the αβγ complex on fibroblast cells. A similar difference was observed with the βγ complex. These observations may indicate functional and constitutional differences of the high‐ and intermediate‐affinity IL‐2 receptor complexes between lymphoid and fibroblastoid cells.

Original languageEnglish
Pages (from-to)2472-2476
Number of pages5
JournalEuropean Journal of Immunology
Volume23
Issue number10
DOIs
Publication statusPublished - 1993 Oct

Keywords

  • High affinity
  • Interleukin‐2
  • Interleukin‐2 receptor
  • γ chain

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Kinetic study of interleukin‐2 binding on the reconstituted interleukin‐2 receptor complexes including the human γ chain'. Together they form a unique fingerprint.

  • Cite this