TY - JOUR
T1 - Kinetic profiles of sequential gene expressions for chemokines in mice with contact hypersensitivity
AU - Mitsui, Gaku
AU - Mitsui, Kazutaka
AU - Hirano, Takeo
AU - Ohara, Osamu
AU - Kato, Masatoshi
AU - Niwano, Yoshimi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/4/3
Y1 - 2003/4/3
N2 - Using cDNA microarray technology, the expression of chemokine genes in the elicitation site of 2,4,6-trinitrochlorobenzene-induced contact hypersensitivity (CHS) was examined in mice. Of the 33 genes analyzed, levels of 11 gene expressions changed, and these can be assigned to four groups based on their kinetic patterns; (1) LARC/CCL20 whose mRNA level increased rapidly at 3 h post-challenge and then gradually decreased, (2) JE/CCL2, MARC/CCL7, MIP-1γ/CCL9, monocyte chemoattractant protein (MCP)-5/CCL12, ELC/CCL19 and BRAK/CXCL14 whose mRNA levels increased with time and reached the maximum at 6-9 h post-challenge, (3) LIX/CXCL5, Mig/CXCL9 and IP-10/CXCL10 whose mRNA levels increased gradually at least up to 12 h post challenge, and (4) SLC/CCL21 whose mRNA level decreased gradually with time after challenge. The findings suggest that sequential expression of chemokine genes is essential for orientating non-specific skin response to hapten-specific CHS response through the recruitment of inflammatory cells such as neutrophils, monocytes/macrophages and T-cells from the circulation into the tissue site.
AB - Using cDNA microarray technology, the expression of chemokine genes in the elicitation site of 2,4,6-trinitrochlorobenzene-induced contact hypersensitivity (CHS) was examined in mice. Of the 33 genes analyzed, levels of 11 gene expressions changed, and these can be assigned to four groups based on their kinetic patterns; (1) LARC/CCL20 whose mRNA level increased rapidly at 3 h post-challenge and then gradually decreased, (2) JE/CCL2, MARC/CCL7, MIP-1γ/CCL9, monocyte chemoattractant protein (MCP)-5/CCL12, ELC/CCL19 and BRAK/CXCL14 whose mRNA levels increased with time and reached the maximum at 6-9 h post-challenge, (3) LIX/CXCL5, Mig/CXCL9 and IP-10/CXCL10 whose mRNA levels increased gradually at least up to 12 h post challenge, and (4) SLC/CCL21 whose mRNA level decreased gradually with time after challenge. The findings suggest that sequential expression of chemokine genes is essential for orientating non-specific skin response to hapten-specific CHS response through the recruitment of inflammatory cells such as neutrophils, monocytes/macrophages and T-cells from the circulation into the tissue site.
KW - CDNA microarray
KW - Chemokine genes
KW - Contact hypersensitivity
UR - http://www.scopus.com/inward/record.url?scp=0037417157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037417157&partnerID=8YFLogxK
U2 - 10.1016/S0165-2478(03)00017-8
DO - 10.1016/S0165-2478(03)00017-8
M3 - Article
C2 - 12644322
AN - SCOPUS:0037417157
VL - 86
SP - 191
EP - 197
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 2
ER -