Kinetic model for synthesis of fructosyl-stevioside using suspended β-fructofuranosidase

Kentaro Suzuki, Takuya Fukumura, Naomi Shibasaki-Kitakawa, Toshikuni Yonemoto

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The synthesis experiments of fructosyl-stevioside were conducted under the various conditions of the initial concentrations of the substrates and the enzyme. The transfructosylation of stevioside with sucrose and the hydrolyses of sucrose and fructosyl-stevioside simultaneously occurred. The fructosyl-stevioside synthesis was inhibited by the side products, glucose and fructose. A kinetic model was constructed by considering the Ping-Pong Bi Bi mechanism for the transfructosylation, the apparent Ordered Uni Bi mechanism for the hydrolysis and the competitive inhibition by the side products. The model constants were estimated by fitting the model equations with the experimental results for the sucrose hydrolysis and the fructosyl-stevioside synthesis. The model can predict not only the appropriate conditions to efficiently synthesize the fructosyl-stevioside, but also the reaction time giving the maximum conversion.

Original languageEnglish
Pages (from-to)207-215
Number of pages9
JournalBiochemical Engineering Journal
Volume10
Issue number3
DOIs
Publication statusPublished - 2002 Apr 13

Keywords

  • Enzymes
  • Fructosyl-stevioside
  • Kinetic parameter
  • Modeling
  • Ping-Pong Bi Bi mechanism
  • Production kinetics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering

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