Kinetic analysis for effect of initial substrate concentration on growth and secondary metabolite production in cultures of Nicotiana tabacum

Namoi Shibasaki, Ryousuke Obika, Toshikuni Yonemoto, Teiriki Tadaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Suspension cultures of tobacco cells were studied using airlift and rotary‐drum bioreactors. The effect of initial concentrations of a major substrate, sucrose, on the growth and production of a secondary metabolite, phenolic compounds, was investigated. The dry weights and total concentrations of the phenolic compounds increased with the initial sucrose concentration in both bioreactors. Both bioreactors were found to have the same tendency for the effect of initial sucrose concentration. The structured model, presented previously was modified by considering that sucrose was hydrolyzed to glucose and fructose by an enzymatic reaction. The previous and the new models were applied to the above two sets of experimental data obtained with two bioreactors, independently. The hydrolysis of sucrose was elucidated to contribute slightly to the overall kinetics of growth and secondary metabolite production in these cultures. Furthermore, the levels of shear damage in each bioreactor were quantitatively compared based on the death rate constant, ki, which is one of the model constants.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalJournal of Chemical Technology & Biotechnology
Volume63
Issue number3
DOIs
Publication statusPublished - 1995 Jul

Keywords

  • Nicotiana tabacum
  • airlift bioreactor
  • product synthesis
  • rotary‐drum bioreactor
  • structured model
  • substrate consumption
  • suspension culture

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'Kinetic analysis for effect of initial substrate concentration on growth and secondary metabolite production in cultures of Nicotiana tabacum'. Together they form a unique fingerprint.

  • Cite this