Junctional adhesion molecule (JAM) is phosphorylated by protein kinase C upon platelet activation

Harunobu Ozaki, Kenji Ishii, Hidenori Arai, Hisanori Horiuchi, Takahiro Kawamoto, Hidenori Suzuki, Toru Kita

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Junctional adhesion molecule (JAM) is a member of the immunoglobulin superfamily (IgSF) expressed in tight junctions of epithelial cells and endothelial cells, and implicated in transendothelial migration of leukocytes. Recently, JAM is reported to be constitutively expressed on circulating monocytes, neutrophils, lymphocytes subsets, and platelets. However, the role of JAM is not known. Here, we examined how phosphorylaton of JAM is regulated upon platelet activation. Phosphorylation of JAM was induced by thrombin, collagen, but not by ADP. The phosphorylated amino acids were shown to be serine residues by phosphoamino acid analysis. Inhibition of JAM's phosphorylation by PKC inhibitors and Ca++ chelator suggests the involvement of conventional types of PKCs. By in vitro kinase assays, we demonstrated that JAM could be directly phosphorylated by cPKCs. We also demonstrated phosphorylation of Ser 284, a putative PKC phosphorylation site, by immunoblotting with anti-phosphoserine-JAM antibody in thrombin-stimulated platelets. In addition to the phosphorylation, JAM seemed to form clusters at several sites of cell-cell contact in aggregated platelets by immunoelectron microscopic study. We speculate that JAM may be directly phosphorylated by cPKC(s) upon platelet activation and that the phosphorylation might be involved in platelet activation. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)873-878
Number of pages6
JournalBiochemical and biophysical research communications
Volume276
Issue number3
DOIs
Publication statusPublished - 2000 Oct 5
Externally publishedYes

Keywords

  • Adhesion
  • Cell-cell contact
  • JAM
  • PKC
  • Phosphorylation
  • Platelet

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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