Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles

Yusuke Sato; Ken Komiya; Ibuki Kawamata; Satoshi Murata; Shin Ichiro M. Nomura

doi:10.1039/c9cc03277k

Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles

Yusuke Sato, Ken Komiya, Ibuki Kawamata, Satoshi Murata, Shin Ichiro M. Nomura

ENG - Robotics

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.

Original language	English
Pages (from-to)	9084-9087
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	62
DOIs	https://doi.org/10.1039/c9cc03277k
Publication status	Published - 2019

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles",

abstract = "An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.",

author = "Yusuke Sato and Ken Komiya and Ibuki Kawamata and Satoshi Murata and Nomura, {Shin Ichiro M.}",

note = "Funding Information: We thank Dr Y. Suzuki for constructive comments and Dr M. Takinoue for providing experimental equipment. This work was supported by JSPS and MEXT KAKENHI (grant numbers JP16J02406, JP15H02774, JP24104003, JP24104004, JP17K18852, and JP18J00720); the ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan; no. 2016-PM14-13-01); and AMED-CREST 19gm0810001h0105. Y. Sato is a JSPS Research Fellow. Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

doi = "10.1039/c9cc03277k",

language = "English",

volume = "55",

pages = "9084--9087",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles

AU - Sato, Yusuke

AU - Komiya, Ken

AU - Kawamata, Ibuki

AU - Murata, Satoshi

AU - Nomura, Shin Ichiro M.

N1 - Funding Information: We thank Dr Y. Suzuki for constructive comments and Dr M. Takinoue for providing experimental equipment. This work was supported by JSPS and MEXT KAKENHI (grant numbers JP16J02406, JP15H02774, JP24104003, JP24104004, JP17K18852, and JP18J00720); the ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan; no. 2016-PM14-13-01); and AMED-CREST 19gm0810001h0105. Y. Sato is a JSPS Research Fellow. Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.

AB - An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.

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SP - 9084

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JO - Chemical Communications

JF - Chemical Communications

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ER -

Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles

Abstract

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