Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands

Taishin Takuma, Akiko Shitara, Toshiya Arakawa, Miki Okayama, Itaru Mizoguchi, Yoshifumi Tajima

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The exocytosis of salivary proteins is mainly regulated by cAMP, although soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which mediate cAMP-dependent exocytic membrane fusion, have remained unidentified. Here we examined the effect of isoproterenol (ISO) and cytochalasin D (CyD) on the level of SNARE complexes in rat parotid glands. When SNARE complexes were immunoprecipitated by anti-SNAP23, the coprecipitation of VAMP2 was significantly increased in response to ISO and/or CyD, although the coprecipitation of VAMP8 or syntaxin 4 was scarcely augmented. These results suggest that the SNAP23-VAMP2 interaction plays a key role in cAMP-mediated exocytosis from parotid glands. Structured summary of protein interactions: Snap23 physically interacts with Vamp8, Vamp3 and Syn-4 by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3) Vamp2 physically interacts with Syn-4, Syn-3 and Snap23 by anti bait coimmunoprecipitation (View interaction) Syn-3 physically interacts with Snap23 by anti bait coimmunoprecipitation (View interaction) Vamp2 physically interacts with Snap23 and Syn-4 by anti bait coimmunoprecipitation (View interaction) Snap23 physically interacts with Vamp8, Syn-4 and Vamp2 by anti bait coimmunoprecipitation (View interaction: 1, 2).

Original languageEnglish
Pages (from-to)583-589
Number of pages7
JournalFEBS Letters
Volume587
Issue number6
DOIs
Publication statusPublished - 2013 Mar 18
Externally publishedYes

Keywords

  • Cytochalasin D
  • Exocytosis
  • Parotid gland
  • SNAP23
  • Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)
  • VAMP2

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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