Isolation of Two Chloroethylnitrosourea-sensitive Chinese Hamster Cell Lines

Hiroaki Hata, Masayuki Ninnata, Hiroko Tohda, Akira Yasui, Atsushi Oikaw

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    18 Citations (Scopus)

    Abstract

    1-[(4-Amino-2-methylpyrimidin-5-yl)methyl]-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU), a cancer chemotherapeutic bifunctional alkylating agent, causes chloroethylation of DNA and subsequent DNA strand cross-linking through an ethylene bridge. We isolated and characterized two ACM-sensitive mutants from mutagenized Chinese hamster ovary cells and found them to be new drug-sensitive recessive Chinese hamster mutants. Both mutants were sensitive to various monofunctional alkylating agents in a way similar to that of the parental cell lines CHO9. One mutant (UVS1) was cross-sensitive to UV and comple mented the UV sensitivity of all Chinese hamster cell lines of 7 estab lished complementation groups. Since UV-induced unscheduled DNA synthesis was very low, a new locus related to excision repair is thought to be defective in this cell line. Another ACNU-sensitive mutant, CNU1, was slightly more sensitive to UV than the parent cell line. CNU1 was cross-sensitive to 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea and slightly more sensitive to mitomycin C. No increased accumulation of ACNU and a low level of UV-induced unscheduled DNA synthesis in this cell as compared with the parental cell line suggest that there is abnormality in a repair response of this mutant cell to some types of DNA cross-links.

    Original languageEnglish
    Pages (from-to)195-198
    Number of pages4
    JournalCancer Research
    Volume51
    Issue number1
    Publication statusPublished - 1991 Jan

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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