Isolation of monoclonal antibodies recognizing rare and dominant epitopes in plant vascular cell walls by phage display subtraction

Naoki Shinohara, Hiroo Fukuda

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A combination of phage display antibody technology and a subtraction method provides a powerful tool for the isolation of novel biomarkers. However, the dilemma that high stringency screening often reduces the diversity in the subtracted phage antibody repertoires and that it is difficult to isolate phage antibody against rare epitopes remain. Therefore, we carefully monitored the occupancy of differentiation-specific clones in a phage antibody library through an enrichment process, and succeeded in isolating monoclonal antibodies against rare and dominant epitopes in plant vascular cell walls. We also report that clones with stop and frameshift mutations significantly survived the enrichment process, owing to noncanonical translation mechanisms.

Original languageEnglish
Pages (from-to)187-194
Number of pages8
JournalJournal of Immunological Methods
Volume264
Issue number1-2
DOIs
Publication statusPublished - 2002 Jun 1
Externally publishedYes

Keywords

  • Complex antigen
  • Differentiation-specific monoclonal antibody
  • Noncanonical translation
  • Phage display subtraction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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