Isolation of monoclonal antibodies recognizing rare and dominant epitopes in plant vascular cell walls by phage display subtraction

Naoki Shinohara, Hiroo Fukuda

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    A combination of phage display antibody technology and a subtraction method provides a powerful tool for the isolation of novel biomarkers. However, the dilemma that high stringency screening often reduces the diversity in the subtracted phage antibody repertoires and that it is difficult to isolate phage antibody against rare epitopes remain. Therefore, we carefully monitored the occupancy of differentiation-specific clones in a phage antibody library through an enrichment process, and succeeded in isolating monoclonal antibodies against rare and dominant epitopes in plant vascular cell walls. We also report that clones with stop and frameshift mutations significantly survived the enrichment process, owing to noncanonical translation mechanisms.

    Original languageEnglish
    Pages (from-to)187-194
    Number of pages8
    JournalJournal of Immunological Methods
    Volume264
    Issue number1-2
    DOIs
    Publication statusPublished - 2002 Jun 1

    Keywords

    • Complex antigen
    • Differentiation-specific monoclonal antibody
    • Noncanonical translation
    • Phage display subtraction

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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