Isolation of mitochondria from Saccharomyces cerevisiae

Toshiaki Izawa, Ann Katrin Unger

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The budding yeast Saccharomyces cerevisiae is an important model organism to study cellular structure and function. Due to its excellent accessibility to genetics and biochemical and microscopic analyses, studies with yeast have provided fundamental insights into mitochondrial biology. Yeast offers additional advantages because it can grow under fermenting conditions when oxidative phosphorylation is not obligatory and because the majority of mitochondrial structure and function are largely conserved during evolution. Isolation of mitochondria is an important technique for mitochondrial studies. This chapter focuses on procedures for the isolation and purification of intact yeast mitochondria that can be used for numerous functional assays as well as for analyses of mitochondrial ultrastructure.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages33-42
Number of pages10
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1567
ISSN (Print)1064-3745

Keywords

  • Cellular fractionation
  • Homogenization
  • Isolation and purification of mitochondria
  • Spheroplasts
  • Yeast culture media

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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