Isolation of inositol hexaphosphate (IHP)-degrading bacteria from arbuscular mycorrhizal fungal hyphal compartments using a modified baiting method involving alginate beads containing IHP

Shintaro Hara, Masanori Saito

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Phytate (inositol hexaphosphate; IHP)-degrading microbes have been suggested to contribute to arbuscular mycorrhizal fungi (AMF)-mediated P transfer from IHP to plants; however, no IHP degrader involved in AMF-mediated P transfer has been isolated to date. We herein report the isolation of IHP-degrading bacteria using a modified baiting method. We applied alginate beads as carriers of IHP powder, and used them as recoverable IHP in the AM fungal compartment of plant cultivation experiments. P transfer from IHP in alginate beads via AMF was confirmed, and extracted DNA from alginate beads was analyzed by denaturing gradient gel electrophoresis targeting the 16S rRNA gene and a clone library method for the beta-propeller phytase (BPP) gene. The diversities of the 16S rRNA and BPP genes of microbes growing on IHP beads were simple and those of Sphingomonas spp. and Caulobacter spp. dominated. A total of 187 IHP-utilizing bacteria were isolated and identified, and they were consistent with the results of DNA analysis. Furthermore, some isolated Sphingomonas spp. and Caulobacter sp. showed IHP-degrading activity. Therefore, we successfully isolated dominant IHP-degrading bacteria from IHP in an AMF hyphal compartment. These strains may contribute to P transfer from IHP via AMF.

Original languageEnglish
Pages (from-to)234-243
Number of pages10
JournalMicrobes and environments
Volume31
Issue number3
DOIs
Publication statusPublished - 2016

Keywords

  • AMF
  • Alginate beads
  • Caulobacter sp
  • Phytate
  • Sphingomonas sp

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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