Isolation of human urinary lysozyme

Nobuhito Masuda, Shizuko Kobayashi, Kensaku Mizuno, Fumio Sakiyama

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

For the isolation of human lysozyme from the urine of leukemia patients, a simple method has been established which involves precipitation of urinary proteins by 60% saturation with ammonium sulfate, fractionation of crude lysozyme on Sephadex G-50 and purification by CM-Sepharose chromatography. By this method approximately 60% of the lysozyme in the urine was isolated in a pure state in ten days.

Original languageEnglish
Pages (from-to)971-975
Number of pages5
JournalJournal of biochemistry
Volume84
Issue number4
DOIs
Publication statusPublished - 1978 Oct
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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