TY - JOUR
T1 - Isolation of human urinary lysozyme
AU - Masuda, Nobuhito
AU - Kobayashi, Shizuko
AU - Mizuno, Kensaku
AU - Sakiyama, Fumio
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1978/10
Y1 - 1978/10
N2 - For the isolation of human lysozyme from the urine of leukemia patients, a simple method has been established which involves precipitation of urinary proteins by 60% saturation with ammonium sulfate, fractionation of crude lysozyme on Sephadex G-50 and purification by CM-Sepharose chromatography. By this method approximately 60% of the lysozyme in the urine was isolated in a pure state in ten days.
AB - For the isolation of human lysozyme from the urine of leukemia patients, a simple method has been established which involves precipitation of urinary proteins by 60% saturation with ammonium sulfate, fractionation of crude lysozyme on Sephadex G-50 and purification by CM-Sepharose chromatography. By this method approximately 60% of the lysozyme in the urine was isolated in a pure state in ten days.
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U2 - 10.1093/oxfordjournals.jbchem.a132211
DO - 10.1093/oxfordjournals.jbchem.a132211
M3 - Article
C2 - 711708
AN - SCOPUS:0018168793
VL - 84
SP - 971
EP - 975
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 4
ER -