Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method

Kripamoy Aguan, Kazuyuki Sugawara, Nobuhiro Suzuki, Tomonobu Kusano

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)

    Abstract

    We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5,-9 and -19. The expression of these genes was slightly stimulated lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rabl6A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lipl9 genes were induced 6 h after such a shift.

    Original languageEnglish
    Pages (from-to)1285-1289
    Number of pages5
    JournalPlant and Cell Physiology
    Volume32
    Issue number8
    Publication statusPublished - 1991 Dec 1

    Keywords

    • Abscisic acid
    • CDNA subtraction
    • Low temperature
    • Oryza sativa

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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