Isolation of genes abundantly expressed in rice anthers at the microspore stage

Tohru Tsuchiya, Kinya Toriyama, Mikhail E. Nasrallah, Shin ichiro Ejiri

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

A cDNA library of rice (Oryza sativa L.) has been constructed from anthers at an early stage of pollen development. By differential screening of the library, we have isolated cDNAs of two genes, designated as Osc4 and Osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. Expression of Osc4 is absent in mature anthers, while Osc6 is present although the expression decays during pollen maturation. A comparison of the nucleotide and deduced amino acid sequences with those in data banks has not shown significant homology to known molecules.

Original languageEnglish
Pages (from-to)1189-1193
Number of pages5
JournalPlant Molecular Biology
Volume20
Issue number6
DOIs
Publication statusPublished - 1992 Dec 1
Externally publishedYes

Keywords

  • anther development
  • cDNA
  • rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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