Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster

Shigeyuki Tsutsui; Yuko Yoshino; Saho Matsui; Osamu Nakamura; Koji Muramoto; Tasuku Watanabe

doi:10.1016/j.fsi.2007.12.001

Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster

Shigeyuki Tsutsui, Yuko Yoshino, Saho Matsui, Osamu Nakamura, Koji Muramoto, Tasuku Watanabe

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.

Original language	English
Pages (from-to)	366-371
Number of pages	6
Journal	Fish and Shellfish Immunology
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/j.fsi.2007.12.001
Publication status	Published - 2008 Mar

Keywords

Conger eel
Epidermis
Immune-mediator
TNF decoy receptor

ASJC Scopus subject areas

Environmental Chemistry
Aquatic Science

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10.1016/j.fsi.2007.12.001

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@article{106dff584e06411db3bfb31f297e49da,

title = "Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster",

abstract = "By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.",

keywords = "Conger eel, Epidermis, Immune-mediator, TNF decoy receptor",

author = "Shigeyuki Tsutsui and Yuko Yoshino and Saho Matsui and Osamu Nakamura and Koji Muramoto and Tasuku Watanabe",

note = "Funding Information: The financial supports of the JSPS Grant-in Aid for Young Scientists (Start Up) 18880025 to S.T. and Grant-in-Aid for Scientific Research (C) 17580172 to O.N. are acknowledged.",

year = "2008",

month = mar,

doi = "10.1016/j.fsi.2007.12.001",

language = "English",

volume = "24",

pages = "366--371",

journal = "Fish and Shellfish Immunology",

issn = "1050-4648",

publisher = "Academic Press Inc.",

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T1 - Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster

AU - Tsutsui, Shigeyuki

AU - Yoshino, Yuko

AU - Matsui, Saho

AU - Nakamura, Osamu

AU - Muramoto, Koji

AU - Watanabe, Tasuku

N1 - Funding Information: The financial supports of the JSPS Grant-in Aid for Young Scientists (Start Up) 18880025 to S.T. and Grant-in-Aid for Scientific Research (C) 17580172 to O.N. are acknowledged.

PY - 2008/3

Y1 - 2008/3

N2 - By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.

AB - By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.

KW - Conger eel

KW - Epidermis

KW - Immune-mediator

KW - TNF decoy receptor

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AN - SCOPUS:39149129963

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SP - 366

EP - 371

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

IS - 3

ER -

Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster

Abstract

Keywords

ASJC Scopus subject areas

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