Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster

Shigeyuki Tsutsui, Yuko Yoshino, Saho Matsui, Osamu Nakamura, Koji Muramoto, Tasuku Watanabe

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.

    Original languageEnglish
    Pages (from-to)366-371
    Number of pages6
    JournalFish and Shellfish Immunology
    Volume24
    Issue number3
    DOIs
    Publication statusPublished - 2008 Mar

    Keywords

    • Conger eel
    • Epidermis
    • Immune-mediator
    • TNF decoy receptor

    ASJC Scopus subject areas

    • Environmental Chemistry
    • Aquatic Science

    Fingerprint Dive into the research topics of 'Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster'. Together they form a unique fingerprint.

    Cite this