Isolation of copper-binding proteins from activated sludge culture

K. Fukushi, S. Kato, T. Antsuki, T. Omura

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Six copper-binding microbial proteins were isolated from activated sludge cultures grown on media containing copper at various concentrations. Molecular weights among isolated proteins were ranged from 1.3k to 174k dalton. Isolated proteins were compared for their copper binding capabilities. Proteins isolated from cultures grown in the presence of copper in the growth media exhibited higher copper binding capabilities than those isolated from the culture grown in the absence of copper. The highest metal uptake of 61.23 (mol copper/mol protein) was observed by a protein isolated from a culture grown with copper at a concentration of 0.25 mM. This isolated protein (CBP2) had a molecular weight of 24k dalton. Other protein exhibited copper binding capability of 4.8-32.5 (mol copper/mol protein).

Original languageEnglish
Pages (from-to)453-459
Number of pages7
JournalWater Science and Technology
Volume44
Issue number2-3
DOIs
Publication statusPublished - 2001

Keywords

  • Activated sludge
  • Chromatography
  • Copper
  • Heavy metals
  • Metal-binding proteins

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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