Isolation of collagen from tiger pufferfish parts and its solubility in dilute acetic acid

Hiroshi Tsukamoto, Yoshihiro Yokoyama, Tohru Suzuki, Shoshi Mizuta, Reiji Yoshinaka, Yoshiaki Akahane

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Collagen was extracted from several tissues (muscle, skin, bone, alimentary tract, gill, fin, hepatopancreas, and air bladder) of the tiger pufferfish Takifugu rubripes, and the content and solubility of the collagen extracted from each tissue were examined. Collagen content in ordinary muscle was 0.95 ± 0.07 % of wet tissue, which is lower than that reported for other fish species even though tiger pufferfish meat has a tough texture. The solubility of collagen extracted from the muscle and skin was relatively high, and collagen accounted for 47.2 ± 7.8 and 70.8 ± 8.1 % of wet tissue, respectively. In contrast, the solubility of the collagen extracted from bone was the lowest of all the tissues examined, being only 5.7 ± 0.8 % of total wet tissue. The extent of hydroxylation of proline and lysine residues was also examined. In most tissues, the extent of hydroxylation of the lysine residue in insoluble collagen was higher than that of acid-soluble collagen, indicating that hydroxylysine contributes to the stability of collagen. This is the first report of collagen contents, solubility, and extent of hydroxylation of proline and lysine residues in collagen extracted from different tissues of one organism. It is possible that hydroxylysine-derived collagen cross-links play a critical role in the stability of collagen in dilute acetic acid.

Original languageEnglish
Pages (from-to)857-864
Number of pages8
JournalFisheries Science
Volume79
Issue number5
DOIs
Publication statusPublished - 2013 Sep 1

Keywords

  • Collagen
  • Fish
  • Hydroxylysine
  • Solubility
  • Takifugu rubripes
  • Tiger pufferfish

ASJC Scopus subject areas

  • Aquatic Science

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    Tsukamoto, H., Yokoyama, Y., Suzuki, T., Mizuta, S., Yoshinaka, R., & Akahane, Y. (2013). Isolation of collagen from tiger pufferfish parts and its solubility in dilute acetic acid. Fisheries Science, 79(5), 857-864. https://doi.org/10.1007/s12562-013-0650-5