Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

We have found a novel type of pluripotent stem cells, Multilineage- differentiating stress enduring (Muse) cells that can be isolated from mesenchymal cell populations. Muse cells are characterized by stress tolerance, expression of pluripotency markers, self-renewal, and the ability to differentiate into endodermal-, mesodermal-, and ectodermal-lineage cells from a single cell, demonstrating that they are pluripotent stem cells. They can be isolated as cells positive for stage-specific embryonic antigen-3, a human pluripotent stem cell marker. Here, we introduce the isolation method for Muse cells and the effect of transplantation of these cells on chronic liver diseases.

Original languageEnglish
Title of host publicationLiver Stem Cells
Subtitle of host publicationMethods and Protocols
EditorsOchiya Takahiro
Pages89-102
Number of pages14
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume826
ISSN (Print)1064-3745

Keywords

  • Chronic liver diseases
  • Muse
  • Pluripotent stem cells
  • SSEA-3
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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