Isolation of a vascular cell wall-specific monoclonal antibody recognizing a cell polarity by using a phage display subtraction method

Naoki Shinohara, Taku Demura, Hiroo Fukuda

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Using a strategy consisting of (i) the isolation of cell walls from synchronously differentiating cells of Zinnia, (ii) the generation of mAbs with an antibody phage display method, and (iii) screening with a subtraction method, we isolated mAbs recognizing vascular development-specific cell wall components without prior antigen identification. One of the isolated mAbs, designated CN 8, recognized a cell wall component contained in the hemicellulosic fraction. Immunohistochemical analyses showed that the CN 8 epitope was localized to the cell wall of immature treachery elements and xylem parenchyma cells. In immature treachery elements, the CN 8 epitope had a polarized localization pattern regardless of whether the cells are formed as parts of vessels in situ or as single treachery elements in vitro, suggesting that cell polarity autonomously formed on the cell wall may function in treachery element differentiation.

    Original languageEnglish
    Pages (from-to)2585-2590
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume97
    Issue number6
    DOIs
    Publication statusPublished - 2000 Mar 14

    ASJC Scopus subject areas

    • General

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