Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams

Nao Takano, Yuko Takahashi, Mitsuru Yamamoto, Mika Teranishi, Hiroko Yamaguchi, Ayako N. Sakamoto, Yoshihiro Hase, Hiroko Fujisawa, Jianzhong Wu, Takashi Matsumoto, Seiichi Toki, Jun Hidema

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    UVB radiation suppresses photosynthesis and protein biosynthesis in plants, which in turn decreases growth and productivity. Here, an ultraviolet-B (UVB)-tolerant rice mutant, utr319 (UV Tolerant Rice 319), was isolated from a mutagenized population derived from 2500 M1 seeds (of the UVB-resistant cultivar 'Sasanishiki') that were exposed to carbon ions. The utr319 mutant was more tolerant to UVB than the wild type. Neither the levels of UVB-induced cyclobutane pyrimidine dimers (CPDs) or (6-4) pyrimidine-pyrimidone photodimers [(6-4) photoproducts], nor the repair of CPDs or (6-4) photoproducts, was altered in the utr319 mutant. Thus, the utr319 mutant may be impaired in the production of a previously unidentified factor that confers UVB tolerance. To identify the mutated region in the utr319 mutant, microarray-based comparative genomic hybridization analysis was performed. Two adjacent genes on chromosome 7, Os07g0264900 and Os07g0265100, were predicted to represent the mutant allele. Sequence analysis of the chromosome region in utr319 revealed a deletion of 45 419 bp. RNAi analysis indicated that Os07g0265100 is most likely the mutated gene. Database analysis indicated that the Os07g0265100 gene, UTR319, encodes a putative protein with unknown characteristics or function. In addition, the homologs of UTR319 are conserved only among land plants. Therefore, utr319 is a novel UVB-tolerant rice mutant and UTR319 may be crucial for the determination of UVB sensitivity in rice, although the function of UTR319 has not yet been determined.

    Original languageEnglish
    Pages (from-to)637-648
    Number of pages12
    JournalJournal of radiation research
    Volume54
    Issue number4
    DOIs
    Publication statusPublished - 2013 Jul 1

    Keywords

    • UVB-tolerance
    • carbon-ion beam
    • rice mutant
    • root bending assay

    ASJC Scopus subject areas

    • Radiation
    • Radiology Nuclear Medicine and imaging
    • Health, Toxicology and Mutagenesis

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