Isolation of a novel PDZ-containing myosin from hematopoietic supportive bone marrow stromal cell lines

Tadashi Furusawa, Shuntaro Ikawa, Nobuaki Yanai, Masuo Obinata

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Stromal cells in bone marrow provide an optimal microenvironment for hematopoiesis. The established stromal cell lines from bone marrow showed various cellular heterogeneities and differed in their hematopoietic supportive ability. By a differential display method, we cloned a gene whose expression levels were correlated with the hematopoietic supportive ability of stromal cells. Its deduced amino acid sequence shows a structure similar to myosins, except that it lacks an actin binding site. Interestingly, it contains a KE-rich sequence and a PDZ domain in the NH2-terminal, which are protein-protein interaction domains; therefore we termed this novel myosin Myosin containing PDZ domain (MysPDZ). Western blot analysis showed that its protein levels positively correlated with the supportive ability of stromal cells and immunostaining suggested that MysPDZ was present at cytoskeleton in a filamentous and/or network form. Thus MysPDZ may be involved in the maintenance of the stromal cell architectures required for cell to cell contact. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalBiochemical and biophysical research communications
Volume270
Issue number1
DOIs
Publication statusPublished - 2000 Apr 2

Keywords

  • Bone marrow stromal cells
  • Hematopoietic supportive ability
  • Myosin
  • PDZ domain

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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