Isolation of β‐galactosidase fractions from Japanese pear: Activity against native cell wall polysaccharides

Yasuhiro Kitagawa, Yoshinori Kanayama, Shohei Yamaki

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

β‐Galactosidase (EC 3.2.1.23) was purified from the cell wall of the fruit of Japanese pear (Pyrus serotina Rehder var. culta Rehder cv. Hosui) and characterized. Five peaks of β‐galactosidase activity, designated as Gal I to V, were separated by hydrophobic chromatography on butyl toyopearl and ion exchange chromatography on Mono S. These isolated β‐galactosidases were investigated with regard to their abilities to release monomeric galactose from the fractionated polymers of native cell wall (cyclo‐hexane‐trans‐1,2‐diamine tetraacetic acid‐, Na2CO3‐, guanidine thiocyanate‐ and KOH‐soluble fractions) and arabinogalactan (from larch wood). All the β‐galactosidase fractions were active against native cell wall polysaccharides although to varying degrees. Gal I reacted to all fractions of native cell wall polysaccharides although to varying degrees. Gal I reacted to all fractions of native cell wall and arabinogalactan. Gal II released much galactose only from KOH‐soluble polymers and arabinogalactan. Gal III released the most galactose. from cyclohexane‐trans‐1,2‐diamine tetraacetic acid‐, Na2CO3‐ and guanidine thiocyanate‐soluble cell wall polymers which probably contained galactosyl side chains of pectic polymers, although it did not react much to arabinogalactan. In addition, the activity of Gal Ill dramatically increased as ripening proceeded. Furthermore, Gal III was purified to homogeneity by gel filtration on TSKgel 3000SW and the size of a polypeptide was 80 kDa on SDS‐PAGE.

Original languageEnglish
Pages (from-to)545-550
Number of pages6
JournalPhysiologia Plantarum
Volume93
Issue number3
DOIs
Publication statusPublished - 1995 Apr
Externally publishedYes

Keywords

  • Cell wall
  • Japanese pear
  • Pyrus serotina
  • pectin
  • ripening
  • softening
  • β‐galactosidase

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Isolation of β‐galactosidase fractions from Japanese pear: Activity against native cell wall polysaccharides'. Together they form a unique fingerprint.

Cite this