TY - JOUR
T1 - Isolation and structural organization of the human preproenkephalin Bgene
AU - Horikawa, Saburo
AU - Takai, Toshiyuki
AU - Toyosato, Mitsuyoshi
AU - Takahashi, Hideo
AU - Noda, Masaharu
AU - Kakidani, Hitoshi
AU - Kubo, Tai
AU - Hirose, Tadaaki
AU - Inayama, Seiichi
AU - Hayashida, Hidenori
AU - Miyata, Takashi
AU - Numa, Shosaku
PY - 1983
Y1 - 1983
N2 - The primary structure of porcine preproenkephalin B has been elucidated by cloning and sequencing cDNA1: it contains neoendorphin2,3, dynorphin4,5 and leumorphin1,6,7 (containing rimorphin as its ammo-terminus)8,9. These opioid peptides, each having a leucine-enkephalin structure10, act on the κ-receptor 7,11-14. We have now cloned a human genomic DNA segment containing the preproenkephalin B gene. The structural organization of this gene resembles those of the genes encoding the other opioid peptide precursors, that is, preproenkephalin A15 and the corticotropin-β-lipotropin precursor16-21 (ACTH-β-LPH precursor). The primary structure of human preproenkephalin B has been deduced from the gene sequence. The amino acid sequence homology observed between preproenkephalin B and preproenkephalin A, together with the similarity between their gene organizations, suggests that the two genes have been generated from a common ancestor by gene duplication.
AB - The primary structure of porcine preproenkephalin B has been elucidated by cloning and sequencing cDNA1: it contains neoendorphin2,3, dynorphin4,5 and leumorphin1,6,7 (containing rimorphin as its ammo-terminus)8,9. These opioid peptides, each having a leucine-enkephalin structure10, act on the κ-receptor 7,11-14. We have now cloned a human genomic DNA segment containing the preproenkephalin B gene. The structural organization of this gene resembles those of the genes encoding the other opioid peptide precursors, that is, preproenkephalin A15 and the corticotropin-β-lipotropin precursor16-21 (ACTH-β-LPH precursor). The primary structure of human preproenkephalin B has been deduced from the gene sequence. The amino acid sequence homology observed between preproenkephalin B and preproenkephalin A, together with the similarity between their gene organizations, suggests that the two genes have been generated from a common ancestor by gene duplication.
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U2 - 10.1038/306611a0
DO - 10.1038/306611a0
M3 - Article
C2 - 6316163
AN - SCOPUS:0021087933
VL - 306
SP - 611
EP - 614
JO - Nature
JF - Nature
SN - 0028-0836
IS - 5943
ER -